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cfx96 touch real time pcr detection system  (Bio-Rad)


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    Bio-Rad cfx96 touch real time pcr detection system
    Cfx96 Touch Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 20188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx+real+time+pcr+system/CFX96+Touch/pm42853894-354-15-21
    Average 99 stars, based on 20188 article reviews
    cfx96 touch real time pcr detection system - by Bioz Stars, 2026-10
    99/100 stars

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    Fluorescence:

    Article Title: Chondroitinase ABC enhances trastuzumab activity via cell-surface chondroitin sulfate cleavage in pancreatic cancer cells.
    Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) has a glycan-rich microenvironment that hampers therapeutic antibody access to a cell-surface target antigen.. The poor outcomes of a clinical trial involving enzymatic degradation of the hyaluronan suggest additional glycan-dependent resistance mechanisms.. In this study, we investigated whether chondroitin sulfate (CS) proteoglycans (CSPGs) enriched in PDAC act as glycan decoy receptors that impair trastuzumab (Tmab) engagement with human epidermal growth factor receptor 2 (HER2).

    Article Title: Wendan tablet and its active component isocurcumenol protect against myocardial ischemia via targeting ALOX15 and inhibiting lipid peroxidation.
    Article Snippet: Background: Wendan Tablet (WDT) is a traditional Chinese herbal compound formulation clinically employed in the management of coronary heart disease (CHD).. Purpose: This study aimed to elucidate the therapeutic mechanism of WDT in treating CHD and to identify its corresponding active constituents.. Methods: The primary components of WDT were identified, and a quantitative analytical method was established for quality control using UHPLC-QTOF-MS. We comprehensively evaluated the cardioprotective effects of WDT against ischemic injury and investigated its role in modulating phospholipid peroxidation of myocardial cells.

    Article Title: Cultivation and Molecular Profiling Reveal Ammonia-Oxidizing Archaea as Skin Commensals.
    Article Snippet: .. DNA was amplified in a CFX Real Time PCR System (Bio-Rad) with following cycling parameters: 95°C for 1 min, 40x cycles of 95°C 15 s denaturation, 60°C 30 s joint annealing-extension, and 60°C 30 s fluorescence measurement. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Chondroitinase ABC enhances trastuzumab activity via cell-surface chondroitin sulfate cleavage in pancreatic cancer cells.
    Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) has a glycan-rich microenvironment that hampers therapeutic antibody access to a cell-surface target antigen.. The poor outcomes of a clinical trial involving enzymatic degradation of the hyaluronan suggest additional glycan-dependent resistance mechanisms.. In this study, we investigated whether chondroitin sulfate (CS) proteoglycans (CSPGs) enriched in PDAC act as glycan decoy receptors that impair trastuzumab (Tmab) engagement with human epidermal growth factor receptor 2 (HER2).

    Article Title: Development of host-mimicking legume-based media for robust induction of sporulation in soybean-associated Cercospora species
    Article Snippet: .. RNA quality and concentration were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). cDNA was synthesized from the extracted RNA using the SuperScriptTMIII First-Strand Synthesis System kit (Thermo Fisher Scientific). qRT-PCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, USA) on a CFX Real-Time PCR System (Bio-Rad), with four biological replicates for each strain and condition. ..

    Article Title: Network toxicology reveals collaborative mechanism of 4NQO and ethanol in esophageal squamous cell carcinogenesis
    Article Snippet: RNA was reverse transcribed into complementary DNA (cDNA) using a reverse transcriptase kit (G3330, Servicebio, China). .. Quantitative real-time PCR was performed with a 2 × SYBR Green qPCR Master Mix (ROX-free) kit (G3320, Servicebio, China) following the manufacturer's protocol on a CFX real-time PCR system (Bio-Rad). ..

    Article Title: Inflammation- and resolution-programmed myeloid circuits govern therapeutic resistance in epithelial and mesenchymal triple-negative breast cancer
    Article Snippet: .. Quantitative real-time PCR was carried out using a CFX Real-Time PCR system (Bio-Rad) and PowerUp SYBR Green Master Mix (Thermo Fisher Scientific). ..

    Article Title: Wendan tablet and its active component isocurcumenol protect against myocardial ischemia via targeting ALOX15 and inhibiting lipid peroxidation.
    Article Snippet: Background: Wendan Tablet (WDT) is a traditional Chinese herbal compound formulation clinically employed in the management of coronary heart disease (CHD).. Purpose: This study aimed to elucidate the therapeutic mechanism of WDT in treating CHD and to identify its corresponding active constituents.. Methods: The primary components of WDT were identified, and a quantitative analytical method was established for quality control using UHPLC-QTOF-MS. We comprehensively evaluated the cardioprotective effects of WDT against ischemic injury and investigated its role in modulating phospholipid peroxidation of myocardial cells.

    Article Title: Genetic and functional characterization of AMH Signaling in Zebrafish - Evidence for Roles of Amh-Bmpr2a-Bmpr1bb Pathway in Controlling Gonadal Homeostasis
    Article Snippet: .. The HRMA was performed on a CFX Real-Time PCR System (Bio-Rad, Hercules, CA), using the following protocol: an initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 sec, a pre-determined optimal annealing temperature of 60°C for 15 sec, and 72°C for 20 sec. A final melting curve analysis, ranging from 70°C to 95°C with increments of 0.2°C at each step, was then performed to distinguish wild-type and mutant alleles based on their distinct melting profiles. .. The data was analyzed using Precision Melt Analysis software (Bio-Rad).

    Article Title: Redox rhythms promote fitness by modulating ageing-dependent reprogramming.
    Article Snippet: Ageing leads to diurnal misalignment with a global reduction in physiological fitness, yet the mechanisms underlying such age-related diurnal reprogramming and its role in ageing remain poorly understood.. H e r e w e generate diurnal transcriptomes across eight peripheral tissues and reveal that disrupted redox oscillations are common diurnal alterations in organismal ageing.. Restoring redox rhythms through the time-restricted application of antioxidants and pro-oxidants markedly improved glucose metabolism, motor performance and ageing-related characteristics of liver and skeletal muscle in male aged mice.

    Article Title: Cultivation and Molecular Profiling Reveal Ammonia-Oxidizing Archaea as Skin Commensals.
    Article Snippet: .. DNA was amplified in a CFX Real Time PCR System (Bio-Rad) with following cycling parameters: 95°C for 1 min, 40x cycles of 95°C 15 s denaturation, 60°C 30 s joint annealing-extension, and 60°C 30 s fluorescence measurement. ..

    Concentration Assay:

    Article Title: Development of host-mimicking legume-based media for robust induction of sporulation in soybean-associated Cercospora species
    Article Snippet: .. RNA quality and concentration were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). cDNA was synthesized from the extracted RNA using the SuperScriptTMIII First-Strand Synthesis System kit (Thermo Fisher Scientific). qRT-PCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, USA) on a CFX Real-Time PCR System (Bio-Rad), with four biological replicates for each strain and condition. ..

    Spectrophotometry:

    Article Title: Development of host-mimicking legume-based media for robust induction of sporulation in soybean-associated Cercospora species
    Article Snippet: .. RNA quality and concentration were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). cDNA was synthesized from the extracted RNA using the SuperScriptTMIII First-Strand Synthesis System kit (Thermo Fisher Scientific). qRT-PCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, USA) on a CFX Real-Time PCR System (Bio-Rad), with four biological replicates for each strain and condition. ..

    Synthesized:

    Article Title: Development of host-mimicking legume-based media for robust induction of sporulation in soybean-associated Cercospora species
    Article Snippet: .. RNA quality and concentration were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). cDNA was synthesized from the extracted RNA using the SuperScriptTMIII First-Strand Synthesis System kit (Thermo Fisher Scientific). qRT-PCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, USA) on a CFX Real-Time PCR System (Bio-Rad), with four biological replicates for each strain and condition. ..

    Quantitative RT-PCR:

    Article Title: Development of host-mimicking legume-based media for robust induction of sporulation in soybean-associated Cercospora species
    Article Snippet: .. RNA quality and concentration were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). cDNA was synthesized from the extracted RNA using the SuperScriptTMIII First-Strand Synthesis System kit (Thermo Fisher Scientific). qRT-PCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, USA) on a CFX Real-Time PCR System (Bio-Rad), with four biological replicates for each strain and condition. ..

    SYBR Green Assay:

    Article Title: Development of host-mimicking legume-based media for robust induction of sporulation in soybean-associated Cercospora species
    Article Snippet: .. RNA quality and concentration were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA). cDNA was synthesized from the extracted RNA using the SuperScriptTMIII First-Strand Synthesis System kit (Thermo Fisher Scientific). qRT-PCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-Rad, USA) on a CFX Real-Time PCR System (Bio-Rad), with four biological replicates for each strain and condition. ..

    Article Title: Network toxicology reveals collaborative mechanism of 4NQO and ethanol in esophageal squamous cell carcinogenesis
    Article Snippet: RNA was reverse transcribed into complementary DNA (cDNA) using a reverse transcriptase kit (G3330, Servicebio, China). .. Quantitative real-time PCR was performed with a 2 × SYBR Green qPCR Master Mix (ROX-free) kit (G3320, Servicebio, China) following the manufacturer's protocol on a CFX real-time PCR system (Bio-Rad). ..

    Article Title: Inflammation- and resolution-programmed myeloid circuits govern therapeutic resistance in epithelial and mesenchymal triple-negative breast cancer
    Article Snippet: .. Quantitative real-time PCR was carried out using a CFX Real-Time PCR system (Bio-Rad) and PowerUp SYBR Green Master Mix (Thermo Fisher Scientific). ..

    Article Title: Redox rhythms promote fitness by modulating ageing-dependent reprogramming.
    Article Snippet: Ageing leads to diurnal misalignment with a global reduction in physiological fitness, yet the mechanisms underlying such age-related diurnal reprogramming and its role in ageing remain poorly understood.. H e r e w e generate diurnal transcriptomes across eight peripheral tissues and reveal that disrupted redox oscillations are common diurnal alterations in organismal ageing.. Restoring redox rhythms through the time-restricted application of antioxidants and pro-oxidants markedly improved glucose metabolism, motor performance and ageing-related characteristics of liver and skeletal muscle in male aged mice.

    Mutagenesis:

    Article Title: Genetic and functional characterization of AMH Signaling in Zebrafish - Evidence for Roles of Amh-Bmpr2a-Bmpr1bb Pathway in Controlling Gonadal Homeostasis
    Article Snippet: .. The HRMA was performed on a CFX Real-Time PCR System (Bio-Rad, Hercules, CA), using the following protocol: an initial denaturation at 95°C for 3 min, followed by 40 cycles of 95°C for 15 sec, a pre-determined optimal annealing temperature of 60°C for 15 sec, and 72°C for 20 sec. A final melting curve analysis, ranging from 70°C to 95°C with increments of 0.2°C at each step, was then performed to distinguish wild-type and mutant alleles based on their distinct melting profiles. .. The data was analyzed using Precision Melt Analysis software (Bio-Rad).

    Amplification:

    Article Title: Cultivation and Molecular Profiling Reveal Ammonia-Oxidizing Archaea as Skin Commensals.
    Article Snippet: .. DNA was amplified in a CFX Real Time PCR System (Bio-Rad) with following cycling parameters: 95°C for 1 min, 40x cycles of 95°C 15 s denaturation, 60°C 30 s joint annealing-extension, and 60°C 30 s fluorescence measurement. ..



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    Journal: iScience

    Article Title: Lateralized vagal oxytocin receptor signaling separately controls feeding and socioemotional functions via hypothalamic oxytocin neurons

    doi: 10.1016/j.isci.2026.117713

    Figure Lengend Snippet:

    Article Snippet: Real-time PCR was performed using the THUNDERBIRD NEXT SYBR qPCR Mix (Toyobo) on a CFX Connect Real-Time PCR system (Bio-Rad, Hercules, CA, USA).

    Techniques: Cell-Signaling, Virus, Recombinant, Software, Imaging, Fluorescence, Real-time Polymerase Chain Reaction